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Symmetric Dimer Formation in Wild Type TIGAR
Two monomer wild type TIGAR molecules were found in dimer structure. Dimer structure is formed through interaction between both beta5 strands. Two Cys201 residues are involved in disulfide bond formation to stabilize the structure. Further dimer stabilization is provided by two potassium (metal) ions bound. Beta5 strands are shown in lightblue. Cys201 and associated disulfide bond is shown in green. Two potassium (metal) ions are shown in space-filling view and in purple.
Hua Li and Gerwald Jogl
Structural and Biochemical Studies of TIGAR (TP53-induced Glycolysis and Apoptosis Regulator The Journal of Biological Chemistry, 284,1748-1754 (PubMed) 3E9D (PDB) |
Dimer Formation in the H11A Mutant TIGAR H11A crystal structure was obtained in the absence of metal ions. As seen, the orientation of two monomers with respect to each other is significantly different than wild type dimer structure. One of the two molecules rotates by almost 180 degrees and previously observed beta strand interaction is absent in this dimer structure. Two Cys201 residues still contribute to disulfide bond formation. However, additional stabilizing forces are absent. Cys201 and associated disulfide bond is shown in green. Overall structure is shown in secondary structure where helices are shown in yellow and beta sheets in orange.
Hua Li and Gerwald Jogl
Structural and Biochemical Studies of TIGAR (TP53-induced Glycolysis and Apoptosis Regulator The Journal of Biological Chemistry, 284,1748-1754 (PubMed) 3E9E (PDB) |